Genome-Wide Characterization of HSP90 Gene Family in Chinese Pumpkin (Cucurbita moschata Duch.) and Their Expression Patterns in Response to Heat and Cold Stresses

Heat shock protein 90 (HSP90) plays critical roles in plant growth and development, as well response to abiotic stresses such heat cold. To comprehensively analyze the HSP90 gene family determine key responsive temperature stress pumpkin (Cucurbita moschata Duch.), bioinformatics molecular biology techniques were used this study. A total of 10 CmoHSP90 genes identified from genome, encoding amino acids 567–865, with weight 64.32–97.36 kDa. Based on phylogenetic analysis, they classified into four groups. The members each group contained similar conserved motifs structures. distributed 9 chromosomes C. moschata. Four pairs segmental duplication (CmoHSP90-1/CmoHSP90-10, CmoHSP90-2/CmoHSP90-7, CmoHSP90-3/CmoHSP90-6, CmoHSP90-4/CmoHSP90-9) detected. Synteny analysis revealed that maxima orthologous 17 syntenic relationships. Promoter detected 23 cis-acting elements including development-, light-, stress-, hormone-related promoter regions genes. Further showed transcript levels CmoHSP90-3 CmoHSP90-6 remarkably up-regulated by stress, while CmoHSP90-10 significantly cold suggesting these play pumpkins. findings will be valuable for understanding CmoHSP90s should provide a foundation elucidating function